A seed of chia (Salvia hispânica L.) has been growing, assuming significant values of consumption in the current society, due to its high nutritional power. However, its seeds can be targeted by the presence of fungi. The aim of this study was to evaluate the health, pathogenicity, and transmission of fungi associated with seeds in chia culture in the southern Tocantins. For the work, chia seeds were collected from the UFT experimental unit, Gurupi-TO, produced from the combination of five levels of phosphate fertilization (0, 30, 60, 90 and 120 kg ha-1) and five levels of nitrogen fertilization, 30, 60, 90 and 120 kg ha-1). The filter paper method (blottertest) was used to perform the mycobacterial survey.The pathogenicity and transmissibility of fungi associated with seeds were also tested. The genus detected in the superficial part of the seeds were: Cladosporiumsp., Phoma sp., Fusarium sp., Alternaria sp. and Curvularia sp. The chia seeds of the municipality of Gurupi-TO presented low incidence of fungi. There was no pathogenicity and transmissibility of the fungi detected in chia seeds in the seedlings.