Hepatitis A Virus Vaccine, Strain HM-175, FI-2, Lot No. 1 of Jan 89 was satisfactorily safety tested in accordance with the guidelines established by the FDA for live and inactivated vaccines.
Topics: DTIC Archive, Potash, Louis, FLOW LABS INC MCLEAN VA, *SAFETY, *HEPATITIS, *VACCINES, LIVE...
Topics: DTIC Archive, JOINT PUBLICATIONS RESEARCH SERVICE ARLINGTON VA, *EPIDEMIOLOGY, TRANSLATIONS,...
Dengue Virus Type 3 (non-attenuated) Strain: CH-53489, LOT No. 1 of April 1984 was satisfactorily safety-tested in accordance with the guidelines established by the FDA for live and inactivated vaccines. Keywords: Dengue-1; Dengue-3; Hepatitis A; Viral seeds; Vaccine.
Topics: DTIC Archive, Potash, Louis, FLOW LABS INC MCLEAN VA, *VACCINES, *DENGUE, STRAINS(BIOLOGY), SAFETY,...
A survey of the transcript levels associated with vaccinia virus was performed. Specific and unique vaccinia promoters were identified by standard techniques and manipulated to drive the expression of foreign genes such as Beta lactosidase and glycoproteins. Promoters of all three classes (early, intermediate, late) were identified and studied. No single promoter was found to be exceptional. Most strong promoters C10LW, H6, 11K, IL3 gave good expression which fell within several fold of each...
Topics: DTIC Archive, Paoletti, Enzo, ALBANY STATE HEALTH LAB NY, *LIVE VACCINES, *VACCINIA VIRUS,...
The purpose of this study was to determine whether a bivalent (dengue-2-4) vaccine could confer immunity in rhesus monkeys against wild type virus infection. It was found that: (1) DEN-2/S-1 animals showed an anamnestic response as well as undetectable viremia after challenge with w.t. DEN-2 virus. Divalent vaccines also showed an anamnestic response after DEN-2 virus challenge. (2) DEN-4 vaccine did not elicit a measurable immune response in 6/8 animals as judged by PRNT, augmented N tests and...
Topics: DTIC Archive, Kraiselburd,E, PUERTO RICO UNIV SAN JUAN, *VACCINES, *DENGUE, *LIVE VACCINES,...
The purpose of this study was to determine how much dengue 2 and dengue 4 viruses were required to infect rhesus monkeys. To this effect, groups of five dengue antibody free animals were inoculated subcutaneously with serial 10 fold dilutions of dengue 2 (PR159) and dengue 4 (H241) viruses. Virus titrations were carried out simultaneously by direct plaque assay in LLC-MK2 cells, by mosquito inoculation using both Aedes aegypti and Toxorynchites amboiensis and in the monkeys. Blood samples for...
Topics: DTIC Archive, Kraiselburd,E, PUERTO RICO UNIV SAN JUAN, *Vaccines, *Live vaccines, *Immunity,...
Dengue Virus Type 4 (CARIB 341750), Challenged Seed, non-attenuated, Lot No. 2 of Jan 1986 was safety tested in accordance with the guidelines established by the FDA for live and inactivated vaccines. The Tissue Culture Purity (Safety) tests were unsatisfactory due to the use of a hyperimmune rabbit antiserum that was subsequently found to readily hemagglutinate guinea pig RBC suggesting the presence in the serum of the a hemagglutinating agent(s). Keywords: Hepatitis A.
Topics: DTIC Archive, Potash, Louis, FLOW LABS INC MCLEAN VA, *DENGUE, *VACCINES, LIVE VACCINES,...
A dengue-2 live virus vaccine was tested in monkeys immune to heterologous dengue serotypes to determine if, as with wild DEN-2 virus, antibody-enhanced viraemia and seroconversion would occur. A low dose of 900 plaque-forming units (PFU) of the DEN-2/S-1 vaccine virus was inoculated subcutaneously into rhesus monkeys six months after they had received wild Den-1, Den-2 or Den-3 viruses, and into non-immune monkeys. As previously reported for non-immune monkeys, these was little, if any,...
Topics: DTIC Archive, Kraiselburd,E, PUERTO RICO UNIV SAN JUAN, *Vaccines, *Live vaccines, *Immunity,...
Four groups of six nonimmune male rhesus monkeys were inoculated subcutaneously with formulations of dengue type 2 vaccine virus DEN-2/S-1. Group A received 1.9 x 104 plague-forming units of vaccine in normal human serum albumin diluent. Group B received the same dose combined with a dengue type 2-immune human serum diluted 1:1,600, beyound its neutralization endpoint of 1:300, but having an immune enhancement titer of 250,000. Groups C and D received 10-fold dilutions of these respective...
Topics: DTIC Archive, Kraiselburd,E, PUERTO RICO UNIV SAN JUAN, *Vaccines, *Live vaccines, *Immunity,...
Previous studies on tularemia prophylaxis have established that live vaccine is far superior to killed vaccines for the immunization of animals and man. Forther investigation indicated, however, that recovery from tularemia provided the monkey even greater immunity. We postulated that a live vaccine prepared from an attenuated mutant selected from a highly virulent North American strain might afford even greater protection than our present vaccine, which contains a mutant derived from an Old...
Topics: DTIC Archive, Eigelsbach, Henry T, ARMY BIOLOGICAL LABS FREDERICK MD, *LIVE VACCINES, *TULAREMIA,...
The safety and immunogenicity of Candid 1, a live-attenuated Junin virus vaccine, were elevated in rhesus macaques. Candid 1 was inoculated subcutaneously in graded doses ranging from 16 to 127,200 plaque-forming units (PFU) into four groups of five animals each; four controls received saline. There was no significant effect of the immunization on any physical, hematologic, or biochemical parameter measured. Junin virus was recovered by cocultivation from peripheral blood mononuclear cells...
Topics: DTIC Archive, McKee, Kelly T , Jr, ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK...
The garnet-type ceramic electrolyte of nominal composition Li7La3Zr2O12 can exist in the tetragonal and cubic form. This article investigates the tetragonal to cubic phase transition based on super-valent cation substitution on the 24c site typically occupied by La (3 ) in the garnet structure. Ce (4 ) was selected as the super-valent cation represented as x in Li7 xLa3 xCexZr2O12. The doping study showed that cubic LLZO was stabilized for Ce 0.2. These data agree with most literature reports...
Topics: DTIC Archive, MICHIGAN STATE UNIV EAST LANSING, *GARNET, *LANTHANUM, *LANTHANUM INTERMETALLICS,...
As a result of the author's experiments, he concludes that: (1) Aerosol immunization of swine against diamond disease by the use of live attenuated cultures of the A70 and VR2 strain under experimental conditions appears to be a safe procedure both for young swine (approximately 35 kg) as well as older (approximately 70 kg); (2) A special resistance is gained, fully protecting 37 of 39 immunized swine from exposure to a powerful intradermal infection carried out 21, 40, and 108 days after...
Topics: DTIC Archive, Wasinski,Konrad, FOREIGN TECHNOLOGY DIV WRIGHT-PATTERSON AFB OHIO, *SKIN DISEASES,...
Hepatitis A Virus, Strain HM175, Vaccine F I-2, Lot 1A of 16 Oct 87 and Lot 1B of 4 Dec 87 was satisfactorily safety-tested in accordance with the guidelines established by the FDA for live and inactivated vaccines as stipulated in 21 CFR, Parts 610.11, 610.12, 630.10-630.17, etc. All testing procedures were carried out following Good Laboratory Practices (GLP) regulations (21 CFR, Part 58). Results are reported herein. Keywords: Vaccines; Laboratory test; Dengue-1; Dengue-3; Hepatitis A; Viral...
Topics: DTIC Archive, Potash, Louis, FLOW LABS INC MCLEAN VA, *HEPATITIS, *VACCINES, STRAINS(BIOLOGY),...
The ultrastructure of the plague bacillus and its relationship to immunity were studied by electron transmission and scanning electron micrsocopic methods. In electron micrographs of colonies, the extracellular material is clearly present after 24 h ofgrowth in virulent strain cultures but not in avirulent strain EV76 (Paris) R. The material did appear in EV76 (Paris) R, however, after 48 h of culture, as well as in once-passaged EV 406. With the addition of Ca(2+) and Mg(2+) to the medium...
Topics: DTIC Archive, Elberg, Sanford S., CALIFORNIA UNIV BERKELEY SCHOOL OF PUBLIC HEALTH, *YERSINIA...
The live vaccine strain (LVS) of Francisella tularensis is the only vaccine against tularemia available for humans, yet its mechanism of protection remains unclear. We probed human immunological responses to LVS vaccination with transcriptome analysis using PBMC samples from volunteers at time points pre- and post-vaccination. Gene modulation was highly uniform across all time points, implying commonality of vaccine responses. Principal components analysis revealed three highly distinct...
Topics: DTIC Archive, Fuller, Claudette L, ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK...
Derivation and characterization of new cold recombinant, AA-CR19, was accomplished. The line was derived by recombination at 25 deg C between the attenuated and temperature sensitive cold variant A/AA/6/60-H3N2. Twenty clones were isolated and analyzed biologically and immunologically. One clone with the desired properties was used to produce an experimental vaccine lot for use in man.
Topics: DTIC Archive, Maassab,H F, MICHIGAN UNIV ANN ARBOR, *INFLUENZA VIRUS, *LIVE VACCINES, LOW...
Marburg virus (MARV) has been associated with sporadic episodes of hemorrhagic fever, including a recent highly publicized outbreak in Angola that produced severe disease and significant mortality in infected patients. MARV is also considered to have potential as a biological weapon. Recently, we reported the development of a promising attenuated, replication-competent vaccine against MARV based on recombinant vesicular stomatitis virus (VSV) expressing the glycoprotein of the Musoke strain of...
Topics: DTIC Archive, Daddario-DiCaprio, Kathleen M, ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT...
Topics: DTIC Archive, NUTTER, JOHN EDWARD, ARMY BIOLOGICAL LABS FREDERICK MD, *IMMUNITY, *RADIATION...
During the past few years a number of experimental studies appeared in Soviet and foreign literature reporting variability phenomena among many species of rickettsiae pathogenic to humans. The present state of the problem of variability of rickettsiae is described thoroughly by P. F. Zdrodovskiy (1959) in his review of Soviet and foreign research. Among the experimental works related to the variability of rickettsiae, those studies deserve the most attention which show the possibility of a...
Topics: DTIC Archive, Genig, V. A., JOINT PUBLICATIONS RESEARCH SERVICE ARLINGTON VA, *USSR, *LIVE...
We are studying the utility of using B.fragilis OmpA as a vehicle on which to put antigenic epitopes of organisms that can be used in bioterror, with the aim of eventually constructing a vaccine vehicle vector. OmpA is the major outer membrane protein of B. fragilis, a gram negative anaerobe that normally resides in the gut. There are four homologs for ompA in the genome. The purpose of this study was to construct a B. fragilis ompA deletant and to begin to characterize the function of OmpA, as...
Topics: DTIC Archive, Wexler, Hannah M, BRENTWOOD BIOMEDICAL RESEARCH INST LOS ANGELES CA, *BACTEROIDES,...
We are studying the utility of using B.fragilis OmpA as a vehicle on which to put antigenic epitopes of organisms that can be used as bioterror agents, with the aim of eventually constructing a vaccine vehicle vector. OmpA is the major outer membrane protein of B. fragilis, a gram negative anaerobe that normally resides in the gut. There are four homologs for ompA in the genome. The purpose of this study was to construct a B. fragilisompA deletant and to begin to characterize the function of...
Topics: DTIC Archive, BRENTWOOD BIOMEDICAL RESEARCH INST LOS ANGELES CA, *ESCHERICHIA COLI, *DISEASE...
A multi-faceted program of applied research has been undertaken in collaboration with the Ministry of Health of Chile intended to lead to control of endemic typhoid fever in Santiago, Chile. Information derived from these studies is directly applicable to the prevention of typhoid fever in United States military personnel deployed in endemic areas. During the past contract year, activities that were emphasized include: Maintenance of prospective epidemiologic and bacteriologic surveillance in...
Topics: DTIC Archive, Levine, Myron M, MARYLAND UNIV BALTIMORE CENTER FOR VACCINE DEVELOPMENT,...
Numerous techniques for the purification of Rickettsiae exist at the present moment. Their high number indicates the extent of the problem. To obtain purified Rickettsiae which are still viable, techniques which involve simple centrifugation in the presence of KCl M/i or, even better, those in which either Amberlit or Sephadex is added to the centrifuged preparation, are recommended. These methods are only slighty more complicated than the technique of Craigie and have the advantage of yielding...
Topics: DTIC Archive, Capponi,M, Giuntini,J, Kawai,K, ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES...
Three healthy young men participating in phase 1 clinical vaccine trials had unexplained increases in their serum transaminase levels. Retrospective analysis indicated that these volunteers had participated in strenuous physical training 2-5 days prior to the noted elevations. The pattern of serum enzyme elevations, initially thought to be consistent with hepatic injury, were associated with parallel increases in creatine phosphokinase. One individual consented to repeat his exercise regimen....
Topics: DTIC Archive, Malinoski, F J, ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD,...
The purpose of this report is to bring to attention the results of investigations dealing with possible cross immunity between L. tropica and L. braziliensis panamensis in an animal model. At this point in time, it would appear that there is some evidence that cross immunity does exist utilizing M. albacaudatus as an animal model. If suitable experimental animals are successfully vacinated with promastigotes of L. tropica (jericho) solid immunity will develop to challenge L. braziliensis or L....
Topics: DTIC Archive, Beacham,Bruce E, VIRGINIA UNIV CHARLOTTESVILLE DEPT OF DERMATOLOGY, *IMMUNITY,...
This study was designed to address a vital concern to our national security: the danger of infectious agents being used vehicles of bioterror. Means to both prevent infection with these agents and to treat infections that may occur are being studied with intensity. Historically, vaccines have been the most efficient method of preventing diseases in large populations, and certainly, in the last decade or so we have seen a precipitous rise in the percentages of multiantimicrobial resistant...
Topics: DTIC Archive, BRENTWOOD BIOMEDICAL RESEARCH INST LOS ANGELES CA, *BACTEROIDES, *BIOLOGICAL AGENTS,...
Production of a live, dry, aerosol anthrax vaccine, from anthrax vaccine strain STI-1 spores, as well as differences from previous methods of production, are described. A 15 minute aerosol immunization session, in the presence of 0.05 g per cu. m. concentration of vaccine is said to insure inhalation of one immunizing dose of anthrax spores. Determination of storage requirements for preservation of spore viability is discussed.
Topics: DTIC Archive, Aleksandrov, N I, ARMY FOREIGN SCIENCE AND TECHNOLOGY CENTER CHARLOTTESVILLE VA,...
We are studying the utility of using B. fragilis OmpA as a vehicle on which to put antigenic epitopes of organisms that can be used in bioterror, with the aim of eventually constructing a vaccine vehicle vector. OmpA is the major outer membrane protein of B. fragilis, a gram negative anaerobe that normally resides in the gut. There are four ompA in the genome. The purpose of this study was to construct a B. fragilis ompA deletant and to begin to characterize the function of OmpA, as well as the...
Topics: DTIC Archive, Wexler, Hannah M, BENTWOOD BIOMEDICAL RESEARCH INST LOS ANGELES CA, *VACCINES,...
Tests of available reduced-virulence auxotrophic mutants of S. typhi showed the aspartate mutant to be ppc, deficient of phosphoenolpyruvate carboxylase; a PAB mutant to be affected at the locus mutated in S. typhimurium mutants pab-501 and pab-503 and mutant 479 to have an early block in purine biosynthesis, at an unidentified locus. Complete blocks in aromatic or purine biosynthesis, or at ppc, resulting from insertion of transposon Tn10 were transduced into two wild-type S. typhi strains;...
Topics: DTIC Archive, Stocker,B A D, STANFORD UNIV CA DEPT OF MEDICAL MICROBIOLOGY, *Live vaccines,...
Topics: DTIC Archive, Pentsik, A. S., JOINT PUBLICATIONS RESEARCH SERVICE ARLINGTON VA, *IN VITRO ANALYSIS,...
The current human plague vaccine, a killed Yersinia pestis whole-cell preparation, does not protect against aerosol challenge and is reactogenic and antigenically undefined. Live attenuated Y. pestis, such as pigmentation-deficient (Pgm-) strains, have been used frequently as vaccines and are efficacious. They are used widely in plague research and assumed to be safe. However, they can cause serious adverse reactions, and their aerosol infectivity is not known. We tested the virulence of a...
Topics: DTIC Archive, Welkos, S, ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD,...
Immune responses after immunization with a number of inactivated Venezuelan equine encephalomyelitis (VEE) virus vaccines were evaluated using an adoptive transfer system. Formalin-inactivated, TC-83 strain VEE virus vaccine was found to be immunogenic and highly effective in protecting recipients against challenge with virulent VEE virus. In contrast to immunization with live, TC-83 VEE virus vaccine, immunization with inactivated vaccine did not provide donors with the capacity to transfer...
Topics: DTIC Archive, Rabinowitz,Stanley G, NORTHWESTERN UNIV CHICAGO ILL MEDICAL SCHOOL, *Immunity,...
Fischer 344 rats were given attenuated live vaccine strain (LVS) of Francisella tularensis by small particle aerosol, intranasal (i.n.) instillation, or by intraperitoneal (i.p.), intramuscular (i.m.), or subcutaneous (s.c.) injection. All of the vaccinated rats developed subclinical infection by 3 days after exposure, which cleared by day 28. Temporal patterns and concentrations of the LVS organisms within the hosts were dependent upon the route a vaccination. Pathologic alterations were...
Topics: DTIC Archive, Jemski,Joseph V, ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FREDERICK MD,...
To evaluate the diagnostic potential of the chemiluminescence (CL) phenomenon for bacterial infections, polymorphonuclear chemiluminescence (PMN CL) was measured following challenge of rats with a gram-negative endotoxin-bearing microorganism, Salmonella typhimurium or Escherichia coli endotoxin. Rats inoculated with a million or a billion live or heat-killed S. typhimurium had a 6-7-fold increase in endogenous PMN CL 24 hr postinjection compared to saline controls. By 72 hr, endogenous PMN CL...
Topics: DTIC Archive, McCarthy,James P, ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FREDERICK MD,...
To evaluate the diagnostic potential of the chemiluminescence (CL) phenomenon for bacterial infections, polymorphonuclear chemiluminescence (PMN CL) was measured following challenge of rats with a gram-negative endotoxin-bearing microorganism, Salmonella typhimurium or Escherichia coli endotoxin. Rats inoculated with a million or a billion live or heat-killed S. typhimurium had a 6-7-fold increase in endogenous PMN CL 24 hr postinjection compared to saline controls. By 72 hr, endogenous PMN CL...
Topics: DTIC Archive, McCarthy,James P, ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FREDERICK MD,...
To evaluate the diagnostic potential of the chemiluminescence (CL) phenomenon for bacterial infections, polymorphonuclear chemiluminescence (PMN CL) was measured following challenge of rats with a gram-negative endotoxin-bearing microorganism, Salmonella typhimurium or Escherichia coli endotoxin. Rats inoculated with a million or a billion live or heat-killed S. typhimurium had a 6-7-fold increase in endogenous PMN CL 24 hr postinjection compared to saline controls. By 72 hr, endogenous PMN CL...
Topics: DTIC Archive, McCarthy,James P, ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FREDERICK MD,...
To evaluate the diagnostic potential of the chemiluminescence (CL) phenomenon for bacterial infections, polymorphonuclear chemiluminescence (PMN CL) was measured following challenge of rats with a gram-negative endotoxin-bearing microorganism, Salmonella typhimurium or Escherichia coli endotoxin. Rats inoculated with a million or a billion live or heat-killed S. typhimurium had a 6-7-fold increase in endogenous PMN CL 24 hr postinjection compared to saline controls. By 72 hr, endogenous PMN CL...
Topics: DTIC Archive, McCarthy,James P, ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FREDERICK MD,...
To evaluate the diagnostic potential of the chemiluminescence (CL) phenomenon for bacterial infections, polymorphonuclear chemiluminescence (PMN CL) was measured following challenge of rats with a gram-negative endotoxin-bearing microorganism, Salmonella typhimurium or Escherichia coli endotoxin. Rats inoculated with a million or a billion live or heat-killed S. typhimurium had a 6-7-fold increase in endogenous PMN CL 24 hr postinjection compared to saline controls. By 72 hr, endogenous PMN CL...
Topics: DTIC Archive, McCarthy,James P, ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FREDERICK MD,...
To evaluate the diagnostic potential of the chemiluminescence (CL) phenomenon for bacterial infections, polymorphonuclear chemiluminescence (PMN CL) was measured following challenge of rats with a gram-negative endotoxin-bearing microorganism, Salmonella typhimurium or Escherichia coli endotoxin. Rats inoculated with a million or a billion live or heat-killed S. typhimurium had a 6-7-fold increase in endogenous PMN CL 24 hr postinjection compared to saline controls. By 72 hr, endogenous PMN CL...
Topics: DTIC Archive, McCarthy,James P, ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FREDERICK MD,...
To evaluate the diagnostic potential of the chemiluminescence (CL) phenomenon for bacterial infections, polymorphonuclear chemiluminescence (PMN CL) was measured following challenge of rats with a gram-negative endotoxin-bearing microorganism, Salmonella typhimurium or Escherichia coli endotoxin. Rats inoculated with a million or a billion live or heat-killed S. typhimurium had a 6-7-fold increase in endogenous PMN CL 24 hr postinjection compared to saline controls. By 72 hr, endogenous PMN CL...
Topics: DTIC Archive, McCarthy,James P, ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FREDERICK MD,...
To evaluate the diagnostic potential of the chemiluminescence (CL) phenomenon for bacterial infections, polymorphonuclear chemiluminescence (PMN CL) was measured following challenge of rats with a gram-negative endotoxin-bearing microorganism, Salmonella typhimurium or Escherichia coli endotoxin. Rats inoculated with a million or a billion live or heat-killed S. typhimurium had a 6-7-fold increase in endogenous PMN CL 24 hr postinjection compared to saline controls. By 72 hr, endogenous PMN CL...
Topics: DTIC Archive, McCarthy,James P, ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FREDERICK MD,...
In order to prevent illnesses in plague endemic regions the USSR is using a live vaccine made on the basis of the EV plague microbe strain. Numerous studies have demonstrated that among the mass methods of plague vaccine immunization, the inhalation method has the greatest productivity, simplicity and is highly effective. At the same time it is known that a single injection of live vaccine, including by inhalation, does not ensure the formation of a lengthy and highly intensive immunity. It is...
Topics: DTIC Archive, Byvalov, A A, ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD,...
To evaluate the diagnostic potential of the chemiluminescence (CL) phenomenon for bacterial infections, polymorphonuclear chemiluminescence (PMN CL) was measured following challenge of rats with a gram-negative endotoxin-bearing microorganism, Salmonella typhimurium or Escherichia coli endotoxin. Rats inoculated with a million or a billion live or heat-killed S. typhimurium had a 6-7-fold increase in endogenous PMN CL 24 hr postinjection compared to saline controls. By 72 hr, endogenous PMN CL...
Topics: DTIC Archive, McCarthy,James P, ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FREDERICK MD,...
To evaluate the diagnostic potential of the chemiluminescence (CL) phenomenon for bacterial infections, polymorphonuclear chemiluminescence (PMN CL) was measured following challenge of rats with a gram-negative endotoxin-bearing microorganism, Salmonella typhimurium or Escherichia coli endotoxin. Rats inoculated with a million or a billion live or heat-killed S. typhimurium had a 6-7-fold increase in endogenous PMN CL 24 hr postinjection compared to saline controls. By 72 hr, endogenous PMN CL...
Topics: DTIC Archive, McCarthy,James P, ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FREDERICK MD,...
To evaluate the diagnostic potential of the chemiluminescence (CL) phenomenon for bacterial infections, polymorphonuclear chemiluminescence (PMN CL) was measured following challenge of rats with a gram-negative endotoxin-bearing microorganism, Salmonella typhimurium or Escherichia coli endotoxin. Rats inoculated with a million or a billion live or heat-killed S. typhimurium had a 6-7-fold increase in endogenous PMN CL 24 hr postinjection compared to saline controls. By 72 hr, endogenous PMN CL...
Topics: DTIC Archive, McCarthy,James P, ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FREDERICK MD,...
Japanese Encephalitis (JE), Strain SA14-14-2 - Production Seed Virus Lot No.PDK8-WR2, Mfg. Date Dec 86 and Live Attenuated Virus Vaccine Lot No. PDK9-WR3, Mfg. Date Feb 87 were satisfactorily safety-tested in accordance with guidelines established by the FDA for live and inactivated vaccines as stipulated in 21 CFR, Parts 610.11, 610.12, 630.10 - 630.18, etc. All testing procedures were carried out following Good Laboratory Practices regulations (21 CFR, Part 58). Keywords: Dengue.
Topics: DTIC Archive, Potash, Louis, FLOW LABS INC MCLEAN VA, *DENGUE, *VACCINES, *VIRUSES, *LABORATORY...
Efforts in this funding year focused on gene gun-based induction of mucosal immune responses and enhancement of CTL. Gene gun-based DNA immunization of rhesus macaques resulted in further refinement of DNA delivery parameters based on the degree of local inflammation. A comparison between mucosal and skin immunization revealed qualitative differences in localized mucosal responses but not peripheral blood responses. Efforts to enhance CTL responses resulted in the development of a gene...
Topics: DTIC Archive, Chen, Dexiang, POWDERJECT VACCINES INC MADISON WI, *DEOXYRIBONUCLEIC ACIDS,...
Immune responses after immunization with a number of inactivated Venezuelan equine encephalomyelitis (VEE) virus vaccines were evaluated using an adoptive transfer system. Formalin-inactivated, TC-83 strain VEE virus vaccine was found to be immunogenic and highly effective in protecting recipients against challenge with virulent VEE virus. Immunization with inactivated vaccine did not provide donors with the capacity to transfer adoptive immunity readily. Only when mice were immunized with...
Topics: DTIC Archive, Rabinowitz,Stanley G, NORTHWESTERN UNIV CHICAGO ILL MEDICAL SCHOOL, *VIRUS DISEASES,...
Temperature-sensitive mutants have been examined to determine their ability to incorporate 3H-uridine into viral specified RNA and complementation patterns. Results of RNA precursor incorporation experiments suggest that mutants may be separated on their ability to synthesize RNA at the nonpermissive temperature. Several mutants have been examined to determine their complementation groups. No complementation has been observed with the very few mutants examined.
Topics: DTIC Archive, Brawner,Thomas A, MISSOURI UNIV-COLUMBIA SCHOOL OF MEDICINE, *Saint Louis...
